Schoedon Gabriele

Group Leader

Prof. Gabriele Schoedon, PhD

 

Name of the Institution

Inflammation Research Unit
Division of Internal Medicine
University Hospital

Street

Rämistr. 100

City

8091 Zürich

Phone

+41-44-255 22 56

E-mail

Group Members

Schaer Dominik, PD, MD, Co-leader
Markus Schneemann, PD, MD, senior scientist
Engelsberger Wolfgang, PhD, postdoctoral scientist
Pereira Claudia, PhD, postdoctoral scientist
Schaer Christian, MD/MSc Med.Biol., research fellow
Vallelian Florence, MD, research fellow
Burgener Julia, MSc Human Biology, research fellow
Kämpfer Theresa, PhD Student
Linnemayr Charlotte, MD-PhD student
Skaria Tom, PhD student
Deuel Jeremy MD, Postgraduate Student for MSc Medical Biologyl
Schaffer Michelle, Master student Human Biology
Lönn Maria, PhD, visiting scientist, Sydney University
Dürst Elenea, BSc ETH Electric Engineering, Student apprentice

Main Field(s) of Research, Abstract

Main objectives of our research are inflammatory processes in macrophages and vascular endothelial cells, macrophage specific anti-inflammatory pathways such as the hemoglobin/hemoglobin-haptoglobin/CD163 pathway, macrophage host factors critical for oppotunistic infections, immunovasular communication, and targets for anti-inflammation Our research aims to the investigation of cytoprotective, anti-inflammatory means for the treatment of acute or chronic inflammatory diseases such as sepsis/septic shock, hemolytic diseases, autoimmune diseases associated with macrophage activation, as well as diseases associated with macrophage dysfunction, especially Whipple’s disease.

Main Fields of Research, Keywords

macrophages, innate immunity, antimicrobial killing systems, CD163, macrophage activation and deactivation, glucocorticoids, cytokines, endothelium, Whipple's disease, hemoglobin toxicity, haptoglobin, hemoglobin scavenging

Special Techniques and Equipment

Fully equipped laboratories for cell and molecular biology techniques. Cell culture lab: culture of primary macrophages from man and mouse, endothelial cells (man, mouse, rat), smooth muscle cells (man, rat) leukemia cells and different cell lines. Immunocytochemistry, microscopy (Zeiss Axiovision System) Equipment for microbial cultures, biohazard level II Molecular biology lab: LightCycler PCR systems (Roche) for research and diagnostics, cloning, differential display, blotting. RNA analysis (RNAse protection assays, RT-PCR, Microarrays). Protein analysis (PAGE, blotting, ELISA). Transcriptome analysis, mass spectrometry based proteome analysis( in collaboration with the FGCZ). Large animal models for investigation of Hb-haptoglobin clearing functions, in collaboration with SwissVet Faculty.

Education and Training

Training opportunities for MD-PhD students and postgraduate MD/Masters in Medical Biology, as well as for Masters in Human Biology.

Selected Publications

Pereira CP, Bachli EB, Schaer DJ, Schoedon G.Transcriptome analysis revealed unique genes as targets for the anti-inflammatory action of activated protein C in human macrophages. PlosOne 2010, 5 (10). E15352.

Vallelian F, Schaer CA, Kaempfer T, Gehrig P, Duerst E, Schoedon G, Schaer DJ. Glucocorticoid treatment skews human monocyte differentiation into a hemoglobin-clearance phenotype with enhanced heme-iron recycling and antioxidant capacity. Blood. 2010 Aug 25. [Epub ahead of print]

Pereira CP, Bachli EB, Schoedon G. The wnt pathway: a macrophage effector molecule that triggers inflammation. Curr Atheroscler Rep. 2009 11(3):236-42.

Boretti FS, Buehler PW, D'Agnillo F, Kluge K, Glaus T, Butt OI, Jia Y, Goede J, Pereira CP, Maggiorini M, Schoedon G, Alayash AI, Schaer DJ. Sequestration of extracellular hemoglobin within a haptoglobin complex decreases its hypertensive and oxidative effects in dogs and guinea pigs. Journal of Clinical Investigation 2009 Aug;119(8):2271-80.

Buehler PW, Abraham B, Vallelian F, Linnemayr C, Pereira CP, Cipollo JF, Jia Y, Mikolajczyk M, Boretti FS, Schoedon G, Alayash AI, Schaer DJ. Haptoglobin preserves the CD163 hemoglobin scavenger pathway by shielding hemoglobin from peroxidative modification. Blood. 2009 Mar 12;113(11):2578-86

Pereira C, Schaer DJ, Bachli EB, Kurrer MO, Schoedon G. Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10. Arterioscler Thromb Vasc Biol. 2008 ;28(3):504-10.

Constitutive endocytosis of CD163 mediates hemoglobin-heme uptake and determines the noninflammatory and protective transcriptional response of macrophages to hemoglobin. Schaer CA, Schoedon G, Imhof A, Kurrer MO, Schaer DJ. Circulation Research. 2006 Oct 27;99(9):943-50. Epub 2006 Sep 28.

Schaer DJ, Schaer CA, Buehler PW, Boykins RA, Schoedon G, Alayash AI, Schaffner A. CD163 is the macrophage scavenger receptor for native and chemically modified hemoglobins in the absence of haptoglobin. Blood. 2006 Jan 1;107(1):373-80.

Franscini N, Bachli EB, Blau N, Leikauf MS, Schaffner A, Schoedon G. Gene expression profiling of inflamed human endothelial cells and influence of activated protein C. Circulation. 2004 ;110(18):2903-9.

Deactivation of macrophages with interleukin-4 is the key to the isolation of Tropheryma whippelii. Schoedon G, Goldenberger D, Forrer R, Gunz A, Dutly F, Höchli M, Altwegg M, Schaffner A. J Infect Dis. 1997 Sep;176(3):672-7. First report of cultivation of the “uncultured bacterium” Tropheryma whipplei

Bone marrow involvement in Whipple's disease: rarely reported, but really rare? Walter R, Bachmann SP, Schaffner A, Rüegg R, Schoedon G. Br J Haematol. 2001 Mar;112(3):677-9.

Marginal and subgingival plaque--a natural habitat of Tropheryma whipplei? Zinkernagel AS, Gmür R, Fenner L, Schaffner A, Schoedon G, Schneemann M. Infection. 2003 Mar;31(2):86-91

Whipple's disease. Bramkamp M, Ruggieri F, Schneemann M. New England Journal of Medicine 2007;356:1480.

Funding

Swiss National Science Foundation, Gianni Rubatto Stiftung, Forschungskredit der Universität Zürich, ZIHP Cooperative Project Grant

URL

Link to "Forschungsdatenbank" of the University