Eberli Daniel

Group Leader

PD Dr. Daniel Eberli, MD, PhD

Name of the Institution

Laboratory for Urologic Tissue Engineering and Stem Cell Therapy
Junior Faculty, Department of Urology
University Hospital Zurich

Street

Frauenklinikstrasse 10

City

8091 Zürich

Phone

+41-44-255 95 49

E-mail

Group Members

Souzan Salemi, PhD, Researcher (Souzan.Salemi@usz.ch)
Maya Horst, MD, Researcher (Maya.Horst@usz.ch)
Ashkan Mortezavi, MD, Researcher (Ashkan.Mortezavi@usz.ch)
Regina Grossmann, MD, Researcher (Regina.Grossmann@usz.ch)
Sarah Nötzli, MSc Human Biology, Research Technican (Sarah.Noetzli@usz.ch)
Deana Haralampieva, PhD Student (Deana.Haralampieva@usz.ch)
Daniel Keller, PhD Student (Daniel.Keller@usz.ch)
Jakub Smolar, PhD Student (Jakub.Smolar@usz.ch)
Oliver Gross, MD Student (Oliver.Gross@usz.ch)

Main Field(s) of Research, Abstract

Tissue engineering (TE), one of the major approaches of regenerative medicine, is a growing and exciting field of research. In combination with better understanding of structure, biology, physiology and cell culture techniques, TE may offer new treatment options for patients needing replacement or repair of an organ. The concept of tissue engineering has been applied clinically for a variety of disorders, for example artificial skin for burn patients, tissue engineered trachea, cartilage for knee-replacement procedures, injectable chondrocytes for the treatment of vesico-ureteric reflux, urinary incontinence and many more.
The principle of classical TE is to dissociate cells from a tissue biopsy, to expand these cells in culture, and to seed them onto the scaffold material in vitro in order to form a live tissue construct prior to implantation into the recipient’s organism. In the appropriate biochemical and biomechanical environment these tissues will achieve their full functional potential and serve as native tissue equivalents. TE products may be fully functional at the time of treatment, or have potential to integrate and form the expected functional tissue after implantation.
The TE approach has major advantages over traditional organ transplantation and does not face the problem of organ shortage. Tissues that closely match the patient’s needs can be reconstructed from a generally readily obtainable biopsy and implanted with minimal or no immunogenicity. This eventually conquers several limitations, encountered in tissue transplantation approaches.
Our research Laboratory is situated within the Department of Clinical Research at the University Hospital of Zürich. Our researchers are applying current scientific knowledge with the ultimate goal to translate this research into clinical treatments and aim at developing future medicine by providing new tailored treatments to patients.
In near future this “personalized medicine” will be individualized by using autologous cells, biomaterials, genetic modifications and bioengineering.
The success in this promising field requires different scientific and clinical specialities. Therefore, we gathered a team with different expertises and backgrounds. The close collaboration within the team and with our collaborators (ETH Zürich) offers the required multidisciplinary dimension to the vision we are embracing.

Ongoing projects

  • Functional innervations of tissue engineered bladder
  • Muscle Precursor Cells (MPCs) and Adipose-derived Stem Cells (ADSCs) for the Treatment of Bladder Voiding Dysfunction
  • Define a reliable cell culture system for human muscle precursor cells (MPC)
  • Tracking muscle precursor cells (MPCs) by MRI
  • Pre-Established Vascular Network for the Engineering of Functional Muscle Tissue
  • Electrospun-Acellular Matrix Composite Scaffold for Bladder Reconstruction
  • Interaction between Adult Stem Cells (ASC) and pre-existing Cancer in Tissue Engineering
  • Pelvic Floor Magnetic Stimulation for the Treatment of Urinary Incontinence in Post-Prostatectomy Patients
  • Donor age and gender limitations for the use of autologous muscle cells
  • Use of Autologous Fat derived Stem Cells for Bladder Regeneration

Main Fields of Research, Keywords

MPCs for Tissue Engineering; Sphincter insufficiency; Stress urinary incontinence; Hinman-Syndrome; Tissue vascularisation; Intestine – Bowel; Innervation; Bladder; Fat derived Stem Cells; Biomaterials – Scaffolds; Adipose derived Stem Cells

Techniques and Equipment

Cell cultures; Immunohistochemistry; Organ Bath; Viral vectors (Adenoviruses); Growth factors; MRI; Infrared sensors; Magnetic Stimulation; Electrospinning

Education and Training

We have training opportunities for research fellows, Postdocs, PhD Students, Master students and medical dissertations

Selected Publications

Schweizer R, Andres A, Galagni M, Giovanoli P, Salemi S, Eberli D, Plock J, Human adipose-derived mesenchymal stromal cells may promote breast cancer progression and , metastatic Spread., Accepted Plastic and Reconstructive Surgery, 2015 (IF 3.3)

Fürer K, Eberli D, Betschart C, Brenneisen R, De Mieri M, Hamburger M, Mennet-von Eiff M, Potterat O, Schnelle M, Simões-Wüst AP, von Mandach U., Inhibition of porcine detrusor contractility by the flavonoid fraction of Bryophyllum pinnatum - a, potential phytotherapeutic drug for the treatment of the overactive bladder syndrome., Phytomedicine. 2015 Jan 15;22(1):158-64. (IF 2.8)

Jaatinen L, Salemi S, Miettinen S, Hyttinen J, Eberli D , The Combination of Electric Current and Copper Promotes Neuronal Differentiation of , Adipose-Derived Stem Cells., Ann Biomed Eng. 2014 (IF 3.2)

Azzabi F, Rottmar M, Virginija J , Rudin M, Sulser T, Boss A, Eberli D, Viability, Differentiation Capacity and Detectability of Super-paramagnetic Iron Oxide Labeled Muscle Precursor Cells for Magnetic Resonance Imaging., Tissue Engineering, 2015 Feb;21(2):182-91 (IF 4.2)

Stoeling M, Hefermehl L, Tremp M, Azzabi F, Susler T, Eberli D, The role of donor age and gender in the success of human muscle precursor cell transplantation., J Tissue Eng Regen Med. 2014 Jul 23 (IF 4.4)

Schweizer R, Känel P, Salemi S, Calcagni M, Giovanoli P, Gorantla VS, Andres AC, Eberli D, Plock J, Bone marrow-derived mesenchymal stem cells improve vascular regeneration and reduce leukocyte-endothelium activation in critical ischemic murine skin in a dose-dependent manner., Cytotherapy. 2014 Jun 24. (IF 3.1)

Azzabi F, Rottmar M, Eberli D, Boss A, MRI relaxometry non-invasively characterizes in-vivo differentiation of muscle precursor cells, Radiology Accepted 2014, (IF: 6.4)

Largo RA, Ramakrishnan V, Marschall J, Ziogas A, Banfi A, Ehrbar M*, Eberli D*, Long-term biostability and bioactivity of fibrin linked VEGF121 in vitro and in vivo., Biomaterial Science. 2014,2, 581-590 (IF Pending)

Benz S, Nötzli S, Siegel JS, Jessen HJ*, Eberli D*, Controlled oxygen release from pyridone endoperoxides promotes cell survival under , anoxic conditions., J Med Chem. 2013 Dec 27;56(24):10171-8 (IF 5.6)

Tremp M, Salemi S, Largo R, Andersson KE, Plock J, Aboushwareb T, Sulser T, Eberli D, Adipose-derived Stem Cells and Muscle Precursor Cells for the Treatment of Bladder , Voiding Dysfunction., World J Urol. 2014 Oct;32(5):1241-8 (IF 3.4)

Horst M , Milleret V, Nötzli S, Madduri S, Gobet R , Sulser T , Eberli D, Increased porosity of electrospun hybrid scaffolds improved bladder tissue regeneration., J Biomed Mater Res A. 2014 Jul;102(7):2116-24 (IF 2.8)

Burnett L, Rahmany MB , Aboushwareb T, Eberli D, Ward C, Orlando G, Hantgan R, Van Dyke M. , Hemostatic properties and the role of cell receptor recognition in human hair keratin protein hydrogels. , Biomaterials. 2013 Apr;34(11):2632-40. (IF 7.4)

Reiner SC, Thiesler T, Eberli D, Klotz E, Frauenfelder T, Sulser T , Moch H, Alkadhi , Computed Tomography Perfusion Imaging of Renal Cell Carcinoma: Systematic Comparison with , Histopathological Angiogenic and Prognostic Markers., Invest Radiol. 2013 Jan 16. (IF 4.6)

Horst M , Madduri S, Hall H, Milleret V, Gobet R , Sulser T , Eberli D, A Bilayered Hybrid Microfibrous PLGA - Acellular Matrix Scaffold for Hollow Organ , Tissue Engineering., Biomaterials. 2013 Feb;34(5):1537-45. (IF 7.4)

Stölting M, Ferrari S, Handshin C, Becskei A, Provenzano M, Sulser T, Eberli D, Myoblasts inhibit tumor growth by paracrine secretion of TNF alpha., J Urol. 2012 Oct 30 (IF 3.7)

Milleret V, Hefti T, Hall H, Vogel V, Eberli D , Influence of fiber diameter and surface roughness of electrospun vascular grafts on , blood activation., Acta Biomater. 2012 Dec;8(12):4349-56. (IF 5.9)

Horst M, Heutschi J, van den Brand R, Andersson KE, Gobet R, Sulser T, Courtine G, Eberli D , Multi-System Neuroprosthetic Training Improves Bladder Function after Severe Spinal , Cord Injury., J Urol. 2012 Sep 13 2012 (IF 3.7)

Schuler V. Fürer K. Eberli D. Horst M. Brenneisen R. Hamburger M. Mennet M. Simoes A.P. von Mandach U., Bryophyllum pinnatum leaf press juice - the effect on the contractility of porcine detrusor in vitro., Phytomedicine. 2012 Jul 15;19(10):947-51 (IF 2.6)

Schlosser S, Dennler C, Schweizer R, Eberli D, Stein JV, Enzmann V, Giovanoli P, Erni D, Plock JA., Paracrine effects of mesenchymal stem cells enhance vascular regeneration in ischemic murine, skin., Microvasc Res. 2012 Feb 25 (IF 3.0)

Eberli D, Aboushwareb T, Soker S, Yoo JJ, Atala A., Muscle Progenitor Cells for the Restoration of Irreversibly Damaged Sphincter Function., Cell Transplant. 2012 Jan 10. (IF 6.3)

Reiner CS, Goetti R, Eberli D, Klotz E, Boss A, Pfammatter T, Frauenfelder T, Moch H, Sulser T, , Alkadhi H., CT Perfusion of Renal Cell Carcinoma: Impact of Volume Coverage on Quantitative Analysis., Invest Radiol. 2011 Jul 1. (IF 4.6)

Chen KL, Eberli D, Yoo JJ, Atala A. , Bioengineered Corporal Tissue For Structural and Functional Restoration of the Penis., Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3346-50 (IF 9.38)

Eberli D, Rodriguez S, Yoo J, Atala A, In vivo evaluation of acellular human dermis for abdominal wall repair., J Biomed Mater Res A. 2010 Jun 15;93(4):1527-38. (IF 2.61)

Stern MM, Myers R, Hammam N, Stern KA, Eberli D, Kritchevskya S, Soker S, Van Dyke M., The influence of extracellular matrix derived from skeletal muscle tissue enhances the proliferation and differentiation of myogenic progenitor cells ex vivo., Biomaterials. 2009 Apr;30(12):2393-9 (IF 6.26)

Eberli D, Soker S, Atala A, Yoo JJ. , Optimization of human skeletal muscle precursor cell culture and myofiber formation in vitro., Methods. 2009 Feb;47(2):98-103 (IF 3.67)

Eberli D, Freitas L, Atala A, Yoo JJ., Composite scaffolds for the engineering of hollow organs and tissues., Methods. 2009 Feb;47(2):109-15 (IF 3.67)

Eberli D, Andersson KE, Yoo JJ, Atala A., A Canine Model of Irreversible Urethral Sphincter Insufficiency., BJU Int. 2009 Jan;103(2):248-53.. (IF 2.75).

Books

“Cells and Biomaterials in Regenerative Medicine“
Edited by Eberli D, ISBN 978-953-51-1731-5, Publication date: December 2014, Hard cover, Publisher Intech

“Regenerative Medicine and Tissue Engineering; Cells and Biomaterials“
Edited by Eberli D, ISBN 978-953-307-663-8, Publication date: August 2011, Hard cover, 588 pages, Publisher Intech

“Tissue Engineering for Tissue and Organ Regeneration“
Edited by Eberli D, ISBN 978-953-307-688-1, Publication date: 2011, Hard cover, 454 pages, Publisher Intech

“Tissue Engineering”
Edited by Eberli D, ISBN 978-953-307-079-7, Publishing date: March 2010, 524 pages, Publisher Intech

Funding

  • Eleonoren-Stiftung and Openheimer Investments
  • “SNF, Schweizer National Fonds” 323230_126230/1
  • EMDO Stiftung
  • Insula Stiftung
  • Sir Allen Standford Pediatric Fellowship Program
  • Matching Fund – University Hospital Zürich
  • Hartmann Müller-Stiftung für medizinische Forschung
  • Stiftung für Forschung an der Medizinischen Fakultät
  • Fonds für Medizinische Forschung
  • Max & Hedwig Niedermeyer Stiftung

URL

Link to "Forschungsdatenbank" of the University

not mentioned